ICSI (Intracytoplasmic Sperm Injection), is a procedure where a single sperm is injected into the egg. To achieve fertilization the sperm is withdrawn into a tiny pipette, the egg fixed with a holding pipette and the sperm injected into the cytoplasm of the metaphase II (mature Oocyte).
After the ICSI procedure the Oocytes are incubated and the resultant embryos are transferred into Uterine cavity by using a specially designed embryo transfer catheter. After the embryo transfer, Progesterone supplementation is given. The confirmation of Pregnancy done with estimation of HCG and after 6 weeks for the foetal heart on ultrasound.
ICSI or Intra-cytoplasmic Sperm Injection is done along with IVF specifically for the cases of male infertility.
- It is majorly done for men with
- Zero sperm count,
- Very low sperm count,
- Poor sperm motility or
- Poor morphology
- Here, the sperm is either collected directly from the testicle, through a surgical procedure or the male is asked to produce a semen sample, then it is washed and one healthy sperm is collected.
- The healthy sperm is then directly injected into one egg and kept in the incubator for fertilization.